Wei Hong Yeo

Wei Hong Yeo

Ph.D. Candidate at Northwestern University

Preprints

Swept-source visible-light optical coherence tomography

Preprint available at Optica Open, 2024

This paper demonstrates the feasibility of swept-source visible-light optical coherence tomography (SS-vis-OCT) using a fanout periodically poled lithium niobate (PPLN) crystal for second harmonic generation (SHG) to convert a commercial near-infrared swept-source (NIR-SS) laser into a visible-light SS laser. I was involved in the initial design and experimental optimization of the SS-vis-OCT system.

Recommended citation: Weijia Fan, Roman Kuranov, David Miller, Tingwei Zhang, Wei-Hong Yeo, Raymond Atkinson, Pengpeng Zhang, Cheng Sun, and Hao F. Zhang (2024). Swept-source visible-light optical coherence tomography. Optica Open. https://doi.org/10.1364/opticaopen.27196476.v1

Implementation and calibration of spectroscopic single-molecule localization microscopy

Preprint available at BioXRiv, 2024

This protocol provides essential information for researchers to implement spectroscopic single-molecule localization microscopy (sSMLM) in a laboratory setting. We describe the assembly and alignment of a 3D dual-wedge prism (DWP)-based sSMLM instrument, spectral and axial calibration, and the reconstruction of super-resolution 3D images using RainbowSTORM v2. I was involved in the development of the MATLAB package, which provides a easy way to reconstruct super-resolution 3D images, as well as the optimization of the calibration procedures.

Recommended citation: Benjamin Brenner*, Wei-Hong Yeo*, Youngseop Lee, Junghun Kweon, Cheng Sun, and Hao F. Zhang (2024). Implementation and calibration of spectroscopic single-molecule localization microscopy. BioXRiv. https://doi.org/10.1101/2024.08.15.608177

Spectroscopic Single-Molecule Discrimination of BODIPY Fluorophores with Deep Learning

Preprint available at Chem, 2024

This paper introduces a library of borondipyrromethene (BODIPY) derivatives for spectroscopic single-molecule imaging, demonstrating the ability to distinguish individual molecules with different substituents using deep learning. I was involved in the acquisition of single-molecule fluorescence data and the development of the deep learning algorithms critical for classification of spectrally similar fluorophores.

Recommended citation: Yeting Zheng*, Wei-Hong Yeo*, Andrea Tomassini, Colin E. Hayter, Hao F. Zhang, Yang Zhang, and Françisco M. Raymo (2024). Spectroscopic Single-Molecule Discrimination of BODIPY Fluorophores with Deep Learning. Chem. http://dx.doi.org/10.2139/ssrn.4888508

Transport and Organization of Individual Vimentin Filaments Within Dense Networks Revealed by Single Particle Tracking and 3D FIB-SEM

Preprint available at BioXRiv, 2024

This paper introduces a sparse vimentin-SunTag labeling strategy to unambiguously visualize individual filament dynamics, revealing extensive bidirectional motion within the perinuclear vimentin network. We also present high-resolution electron microscopy volumes of a vitreously frozen cell to reconstruct VIFs and microtubules within a ∼50 µm3 window, demonstrating minimal local co-alignment between VIFs and microtubules. I was involved in the image analysis and interpretation of the single-particle tracking data.

Recommended citation: Bhuvanasundar Renganathan*, Andrew Moore*, Wei-Hong Yeo, Alyson Petruncio, David Ackerman, Aubrey Wiegel, The CellMap Team, H. Amalia Pasolli, C. Shan Xu, Gleb Shtengel, Harald F. Hess, Anna S Serpinskaya, Hao F. Zhang, Jennifer Lippincott-Schwartz, and Vladimir I. Gelfand (2024). Transport and Organization of Individual Vimentin Filaments Within Dense Networks Revealed by Single Particle Tracking and 3D FIB-SEM. BioXRiv. https://doi.org/10.1101/2024.06.10.598346

Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms

Preprint available at BioRxiv, 2024

This paper introduces an improved dual-wedge prism-based monolithic imaging spectrometer for spectroscopic single-molecule localization microscopy (sSMLM). I was involved in the experimental design, validation, and data analysis of the new imaging system, which demonstrated improved spatial and spectral precisions compared to grating-based sSMLM.

Recommended citation: Wei-Hong Yeo, Benjamin Brenner, Youngseop Lee, Junghun Kweon, Cheng Sun, and Hao F. Zhang (2024). Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms. BioRxiv. https://doi.org/10.1101/2024.05.12.593746

Publications

Dynamic chromosome association with nuclear organelles in living cells

Published in Histochem Cell Biol, 2024

This paper investigates the dynamic nature of chromosomes in relation to nuclear organelles across the cell cycle using photoconvertible GFP H3-Dendra2 in PC3M cells. I was involved in the analysis of chromosome dynamics in living cells.

Recommended citation: Lam Minh Uyen Phan*, Wei-Hong Yeo*, Hao F. Zhang, and Sui Huang (2024). Dynamic chromosome association with nuclear organelles in living cells. Histochem Cell Biol, 162, 149–159. https://doi.org/10.1007/s00418-024-02288-8

Longitudinal imaging of vitreal hyperreflective foci in mice with acute optic nerve damage using visible-light optical coherence tomography

Published in Optics Letters, 2024

This paper demonstrates the use of visible-light optical coherence tomography (vis-OCT) with temporal speckle averaging to visualize and track vitreal hyperreflective foci (VHRFs) in a mouse model of acute optic nerve damage. I was involved in the analysis of the vis-OCT images, which revealed a significant increase in VHRF density following optic nerve crush injury.

Recommended citation: Weijia Fan, David A. Miller, Shichu Chang, Junghun Kweon, Wei-Hong Yeo, Marta Grannonico, Xiaorong Liu, and Hao F. Zhang (2024). Longitudinal imaging of vitreal hyperreflective foci in mice with acute optic nerve damage using visible-light optical coherence tomography. Optics Letters, 49, 1880-1883. https://doi.org/10.1364/OL.512029

Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy

Published in Journal of Biomedical Optics, 2023

This paper combines spectroscopic single-molecule localization microscopy (SMLM) with a Monte Carlo (MC) simulation to effectively model the imaging process with a dual-wedge prism (DWP), enabling us to better optimize our experimental setups for improved spectroscopic performance. I was involved in the development of the MC model, the optimization of the DWP system parameters, and the validation of the simulation results.

Recommended citation: Wei-Hong Yeo, Cheng Sun, and Hao F. Zhang (2023). Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy. Journal of Biomedical Optics. https://doi.org/10.1117/1.JBO.29.S1.S11502

Investigating uncertainties in single-molecule localization microscopy using experimentally informed Monte-Carlo simulation

Published in Nano Letters, 2023

This paper combines single-molecule localization microscopy (SMLM) with a Monte Carlo (MC) simulation to effectively model the labeling uncertainties of the nuclear pore complex (NPC), enhancing the interpretation of SMLM images and deepening our understanding of cellular substructures. I was involved in the development of the MC model, the optimization of the clustering algorithm, and the generation of synthetic SMLM images.

Recommended citation: Wei-Hong Yeo, Yang Zhang, Amy E. Neely, Xiaomin Bao, Cheng Sun, and Hao F. Zhang (2023). Investigating uncertainties in single-molecule localization microscopy using experimentally informed Monte-Carlo simulation. Nano Letters, 23(16), 7253-7259. https://doi.org/10.1021/acs.nanolett.3c00852

Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy

Published in Nanophotonics, 2022

This paper introduces a dual-wedge prism-based monolithic imaging spectrometer for spectroscopic single-molecule localization microscopy (sSMLM), which offers improved performance and feasibility compared to traditional grating-based methods. I was involved in the experimental validation of the dual-wedge prism, which demonstrated improved spatial and spectral precisions compared to grating-based sSMLM.

Recommended citation: Ki-Hee Song, Benjamin Brenner, Wei-Hong Yeo, Junghun Kweon, Zhen Cai, Yang Zhang, Youngseop Lee, Xusan Yang, Cheng Sun, and Hao F. Zhang (2022). Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy. Nanophotonics, 11(8), 1527–1535. https://doi.org/10.1515/nanoph-2021-0541

*Co-first authorship.